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Thyna makes it possible to obtain substantially better results compared to conventionallly used standard sequencing techniques

Thyna was developed by VITO to turn micro arrays into more precise tools. By expanding the hybridisation theory of a two-state model to a three-state model, much better results can be obtained than by using standard sequencing techniques.

What all DNA microarrays have in common is the basic underlying reaction of hybridization between a nucleic acid strand in solution (target) and a complementary strand linked covalently at a solid surface (probe).

Hybridization is characterized by a sequence dependent free energy difference ΔG which measures the binding affinity for the two strands forming a duplex. In current microarray experiments it is assumed that the hybridization reaction has reached equilibrium when the optical read-out is performed. This theory assumes that the hybridization process is a two-state process - either hybridization has  taken place (bound state) or it has  not  (unbound state).

Nearest neighbor models provide reasonable approximation of the free energy difference for strands hybridization in solution. Such models estimate the hybridization free energy as a sum of dinucleotide parameters. These parameters are then fitted through a series of (labour intensive) experiments. The relationship between hybridization in solution and hybridization in DNA microarrays is nevertheless still not clear.
 

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