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DHYNA makes widening of the dynamic range of a hybridization experiment possible

Dhyna enables a much greater dynamic range of experiments relating to hybridisation analysis.  This allows for samples to be analysed under less rigorous conditions than is the case with conventional methods.

The accurate detection of DNA sequence variations such as single nucleotide variations (SNVs) and somatic mutations is of great interest for a variety of research domains. For example, single nucleotide polymorphisms (SNPs) are DNA sequence variations between members of the same species characterized by a single nucleotide difference and account for the majority of the genome variations between individuals of said species.

Techniques for the detection of sequence variations such as SNPs can mainly be classified as either hybridization-based techniques or enzyme-based techniques such as PCR and sequencing. The use of hybridization-based techniques is appealing due to their inherent simplicity. These techniques typically involve providing a nucleic acid probe to hybridize to the perfectly matching DNA target with a high efficiency, while having a low efficiency for targets containing a single nucleotide variation.

In current practice, hybridization analysis involves the analysis of hybridization intensities for different probes as a function of hybridization free energy.

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